Minnebio develops and manufactures enzymes, cytokine growth factors, and cell culture media for use in academic laboratory and pharmaceutical companies; and also provides services to develop and prepare customized proteins and cell culture media.
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  • Deoxyribonuclease I (DNase I)

Deoxyribonuclease I (DNase I)

SKU:
$70.00
70 718 $70.00 - $718.00
Unavailable
per item
Products forms: Both liquid and lyophilized 
Pack sizes: 1000 U, 3000 U and 10 kU
Storage: -20 °C
Molecular weight: 29 kDa
Source: Bovine DNase I from E Coli
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DNase I (EC: 3.1.21.1), is a recombinant endonuclease that cleaves both single- and double-stranded DNA preferentially at phosphodiester linkages adjacent to pyrimidine nucleotides, producing oligodeoxyribonucleotides with 5'-phosphate and 3'-OH ends. The catalytic activity of DNase I is dependent on metal ions including structural calcium Ca2+ and catalytic magnesium Mg2+ and manganese Mn2+. In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently and randomly. In the presence of Mn2+, DNase I cleaves each strand of dsDNA at approximately the same sites.

Applications:
  • It is typically used for selectively degrading unwanted DNA in the presence of RNA in the samples.
  • DNase I foot printing assay for identification of the precise DNA sequence bound by the protein of interest
  • Removing genomic DNA from RNA samples prior to sensitive applications such as RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction), and Northern blotting
  • Removing DNA template after in vitro transcription
  • Radioactive or fluorescent labeling: digesting the radioactively labeled or fluorescently labeled DNA fragment, and then uses gel electrophoresis to detect the resulting cleavage pattern.
  • DNA removal in bioprocessing applications, like DNA removal from cell lysates to decrease viscosity providing better yields
  • Applying to experiments like Nick Translation, Actin binding, UV crosslinking of proteins to nucleic acids, and investigation on chromatin
  • Digesting DNA in the tissue dissociation medium from cell damage.

Technical notes:
  • Purity >98% by Coomassie Blue Staining
  • Specific activity 5000/1000 U/mg or 5000/1000 U U/ml
  • Endotoxin ≤ 0.1 EU/ml (0.0001 EU/ 1000 U)
  • Unit Definition: One unit is defined as completely degrades 1 μg of DNA substrate in 10 min at 37 oC in the reaction buffer.
  • Reaction buffer: 50 mM Tris-HCl in at pH 8 with 2 mM MgCl2 and 5 mM CaCl2.
  • Storage and Shipping Stored in the buffer of 50 mM Tris-HCl in 50% glycerol at pH 8 with 2 mM MgCl2 and 5 mM CaCl2.

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  • Home
  • Products
    • ​​PROTEINASE & PROTEASE >
      • Ultra-active Nuclease, Benzonase
      • Deoxyribonuclease 1
      • HRV 3C Protease
      • Protease K
    • Cytokine Growth Factor >
      • Fibroblast growth factor-2, FGF-2
      • Interleukin-2, IL-2
  • Lab Services
  • Advisor
  • About Us
  • Contact Us
  • Lab Supply