Fibroblast growth factor FGF2, also known as basic fibroblast growth factor is a growth factor that is involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF2 regulates cellular behavior through binding to fibroblast growth factor receptors 1, 2, 3 and 4 (FGFR1-4) inducing intracellular signaling pathways. FGF-2 requires cofactors present in the extracellular matrix to regulate the cell proliferation in vivo. The interaction of FGF-2 with cell surface heparin sulfate is essential for the binding and activation of FGF receptors. Heparan sulfate/heparin promotes FGF and its receptor FGFR to assemble into a functional dimer on the cell surface and further induces downstream cell signaling. FGF-2 is a critical component of stem cell culture medium
that maintains the stem cells undifferentiated.
Applications:
FGF2, as growth factor has been applied for cell culture and for the study of signaling pathways.
Technical notes:
that maintains the stem cells undifferentiated.
Applications:
FGF2, as growth factor has been applied for cell culture and for the study of signaling pathways.
- As a supplement in the neurobasal culture medium
- Preventing endothelial cells from undergoing cell death and promoting endothelial cell proliferation and angiogenesis
- Used for maintenance of the undifferentiated state in stem cell culture
Technical notes:
- Purity >98% by Coomassie Blue Staining
- ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of BALB/c 3T3 cells in a dose dependent manner.
- Endotoxin ≤ 0.1 EU/ml (0.0001 EU/ 1000 U)
- Storage and Shipping Stored in PBS buffer. FGF-2 was also lyophilized from the PBS buffer. Reconstitute with dH 2 O to a concentration not less than 0.1 mg/ml and further dilute with a desired buffer.
- FGF-2 can be stored in working aliquots at 4 °C for 1-2 weeks, or at -20°C for 2-3 months with BSA. Avoid repeated freeze/thaw cycles. The lyophilized protein is stable for at least
one year at -80°C.