Minnebio develops and manufactures enzymes, cytokine growth factors, and cell culture media for use in academic laboratory and pharmaceutical companies; and also provides services to develop and prepare customized proteins and cell culture media.
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Fibroblast growth factor-2, FGF-2

SKU:
$100.00
100 680 $100.00 - $680.00
Unavailable
per item

Products formats: Both liquid and lyophilized

powder

Pack sizes: 100 µg, 250 µg and 1 mg

Storage: -20 °C lyophilized/4 °C liquid

Molecular weight: 16.4 kDa

Source: E Coli

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Fibroblast growth factor FGF2, also known as basic fibroblast growth factor is a growth factor that is involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF2 regulates cellular behavior through binding to fibroblast growth factor receptors 1, 2, 3 and 4 (FGFR1-4) inducing intracellular signaling pathways. FGF-2 requires cofactors present in the extracellular matrix to regulate the cell proliferation in vivo. The interaction of FGF-2 with cell surface heparin sulfate is essential for the binding and activation of FGF receptors. Heparan sulfate/heparin promotes FGF and its receptor FGFR to assemble into a functional dimer on the cell surface and further induces downstream cell signaling. FGF-2 is a critical component of stem cell culture medium
that maintains the stem cells undifferentiated. 

Applications:
FGF2, as growth factor has been applied for cell culture and for the study of signaling pathways.
  • As a supplement in the neurobasal culture medium
  • Preventing endothelial cells from undergoing cell death and promoting endothelial cell proliferation and angiogenesis 
  • Used for maintenance of the undifferentiated state in stem cell culture

Technical notes:
  • Purity >98% by Coomassie Blue Staining
  • ED50 ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1.0 x 107 units/mg, as measured by its ability to stimulate proliferation of BALB/c 3T3 cells in a dose dependent manner.
  • Endotoxin ≤ 0.1 EU/ml (0.0001 EU/ 1000 U)
  • Storage and Shipping Stored in PBS buffer. FGF-2 was also lyophilized from the PBS buffer. Reconstitute with dH 2 O to a concentration not less than 0.1 mg/ml and further dilute with a desired buffer.
  • FGF-2 can be stored in working aliquots at 4 °C for 1-2 weeks, or at -20°C for 2-3 months with BSA. Avoid repeated freeze/thaw cycles. The lyophilized protein is stable for at least
    one year at -80°C.​

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  • Home
  • Products
    • ​​PROTEINASE & PROTEASE >
      • Ultra-active Nuclease, Benzonase
      • Deoxyribonuclease 1
      • HRV 3C Protease
      • Protease K
    • Cytokine Growth Factor >
      • Fibroblast growth factor-2, FGF-2
      • Interleukin-2, IL-2
  • Lab Services
  • Advisor
  • About Us
  • Contact Us
  • Lab Supply